| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Centennial |
A | 8:47.02 | Royal Palm Beach Meet 2025 |
| 2 | Vero Beach |
A | 8:55.00 | |
| 3 | Fort Pierce Central |
A | 8:55.51 | 28th Annual Warrior Invitational 2024 |
| 4 | Lincoln Park |
A | 9:08.91 | FHSAA 2A District 8 |
| 5 | Sebastian River |
A | 9:15.00 | |
| 6 | Jensen Beach |
A | 9:21.27 | Spanish River Invitational 2025 |