| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fort Pierce Westwood |
A | Blaise | Brown | Moody | Holmes | NT | |
| 4 | Lincoln Park |
A | Anderson | Huskin | Whilby | Sanchez-Appell | 4:39.51 | Cobra Relays 2026 |
| 5 | Fort Pierce Central |
A | Simeus | Alexander | Spence | Brown | 5:14.29 | MCHS Preseason PR Carnival |
| 6 | John Carroll |
A | Tokman | ANDERSEN | PEREZ | Russell | NT |