| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Space Coast |
A | Kemp | Pugh | Parson | Terronez | NT | |
| 2 | Fort Pierce Westwood |
A | DOUGLAS | Banner | Stubbs | Williams | 4:19.54 | Cobra Relays 2026 |
| 3 | Fort Pierce Central |
A | Flowers | Nathan-Marsh Jr | Phanor | Pascoe | 4:07.54 | MCHS Preseason PR Carnival |
| 4 | Vero Beach |
A | Dagbolen | Mitchell | Lundy | Williams | 4:01.00 | Vero Beach HS Relay Championships (FHSAA Sactioned) |
| 5 | John Carroll |
A | DAVIS JR | RICHARDSON | Housing | MAURICIO | 4:05.16 | Cobra Relays 2026 |
| 6 | Lincoln Park |
A | Swaby-Clacken | Chagani | Cunningham | Luc | 4:15.32 | Cobra Relays 2026 |
| 7 | Sebastian River |
A | Gibson | Sicard | Carilus | Morel | NT |