| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | CADLE, Janelle | Heritage | NT | NT |
| 4 | MESSAM, Alexis | Vero Beach | NT | NT |
| 5 | WALKER, Aleena | Edgewood | NT | NT |
| 6 | EVANS, Cetirria | Vero Beach | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BUSTILLO, Anais | Edgewood | 25.82 | Merritt Island Mini-Meet |
| 2 | CARTER, Simone | Melbourne | 21.61 | St. Cloud Invitational |
| 3 | BLACKBURN, Isabella | Edgewood | 21.30 | Merritt Island Mini-Meet |
| 4 | KIRTON, Andreanna | Heritage | 20.16 | FHSAA 3A District 13 |
| 5 | PALMER, Janessa | Melbourne | 20.76 | St. Cloud Invitational |
| 6 | STEFFEN, Emma | Sebastian River | 21.49 | Harmony Invitational |
| 7 | JOHNSON, Arianna | Melbourne | 22.18 | Satellite HS Scorpion Invitational 2023 |
| 8 | GEORGE, Nora | Eau Gallie | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SMART, Tevenese | Centennial | 19.87 | Jeff Fossen Memorial Meet 2023 |
| 2 | ALDER, Shayna | Heritage | 19.74 | St. Cloud Invitational |
| 3 | ALEXIS, Rashelle | Sebastian River | 18.72 | Treasure Lake Conference |
| 4 | RUTHERFORD, Paige | Viera | 17.54 | Holy Trinity Invitational |
| 5 | PORTER, Aniya | Fort Pierce Westwood | 17.58 | Cobra Relays 2023 |
| 6 | CATALINI, Silvia | Vero Beach | 19.49 | Satellite HS Scorpion Invitational 2023 |
| 7 | JONES, Journey | Lincoln Park | 19.85 | South Fork 2022 |
| 8 | AHMAD, Saleemah | Sebastian River | 20.16 | Cobra Relays 2023 |