| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jupiter Christian |
A | Garcia | WAYCHOWSKY | Fielder | Winslow | 4:32.26 | FHSAA 1A District 13 |
| 4 | Benjamin School |
A | Foster | Woods | Marshall | Lewis | 3:59.95 | FHSAA State Track & Field Championships (1A) |
| 5 | St. Edward's |
A | Callaghan | Hebig | Storts | Perrin | 4:30.06 | FHSAA 1A Region 4 |
| 6 | Treasure Coast Classical | A | Kellerman | Burns | Wolf | Griffin | DNS | MCHS Fesh/Soph 2025 |