| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jupiter |
A | Ulmer | Godinho | O'Shea | Hanssen | 10:24.25 | Dr. Garcia Invite #2 2025 |
| 2 | St. Edward's |
A | Callaghan | Cooper | Hebig | Perrin | 10:52.47 | Benjamin Invite #1 2025 (Sanctioned) |
| 3 | Treasure Coast Classical | A | Goers | Wojcieszak | Hook | Goers | NT |