| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jupiter Christian |
A | WEDA | FLORA | Evans | Mirabella | 3:59.30 | Pine Crest Panther Relays |
| 4 | Benjamin School |
A | Sukhu | Ryba | Arwood | Winfield | 3:19.90 | FHSAA State Track & Field Championships (1A) |
| 5 | St. Edward's |
A | Blackman | Leibik | Taft | Colontrelle | 3:54.61 | Viera High School Invitational |