| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jupiter |
A | Ward | Diaz | Prasade | O'Shea | 8:43.77 | Dr. Garcia Invite #2 2025 |
| 2 | St. Edward's |
A | Blackman | Bryant | Perrin | Perrin | 9:25.31 | Benjamin Invite #1 2025 (Sanctioned) |
| 3 | St. John Paul II |
A | Hernaiz | Dohler | Coleman | Matheus | 10:45.00 | |
| 4 | Benjamin School |
A | Bradley | Irish | Foster | Klein | 12:27.00 |