| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jupiter Christian |
A | Reynolds | Carrol | Garcia | MUNIZ | 4:40.99 | Calvary Christian FTL Varsity Meet #1 |
| 4 | Benjamin School |
A | Arendts | Sukhu | Gay | Travers | 4:03.60 | 2026 PBC County Championships |
| 5 | Treasure Coast | A | Smith | Smith | Sama | Hatch | DNS | 2026 St. Lucie County Championships |