| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | SORIANO, Isabella | Suncoast | NT | NT |
| 5 | MORGAN, Alysia | Suncoast | 1:29.51 | NPB Conference Meet 2023 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROSENSTEIN, Sophie | St. Edward's | 1:15.46 | Martin County - Tiger Invite |
| 2 | NELMS, Danielle | Berean Christian | 1:13.62 | Westminster Academy Invitational |
| 3 | REDCROSS, Leyla | Olympic Heights | 1:10.77 | Benjamin Meet #1 2023 |
| 4 | FOGA, Jessica | Suncoast | 1:09.09 | PBGHS Gator Invitational 2023 |
| 5 | HAAKE, Emma | Jupiter | 1:10.09 | Jupiter Preseason 2023 |
| 6 | MORALES, Jeniya | Olympic Heights | 1:13.52 | Benjamin Meet #1 2023 |
| 7 | ANDREWS, Mariam | Berean Christian | 1:14.54 | Jeff Fossen Memorial Meet 2023 |
| 8 | SALAZAR, Daniella | Olympic Heights | 1:15.69 | Kings Var Open 2 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JONES, Anna | Olympic Heights | 1:07.57 | Atlantic High School Conference Meet |
| 2 | JOHNSON, Aria | Jupiter | 1:05.27 | Warrior Invitational 2023 |
| 3 | INNOCENT, Dylande | Berean Christian | 1:02.90 | FHSAA 1A Region 4 |
| 4 | CHRISTIAN, Chelsea | Berean Christian | 59.48 | Westminster Academy Invitational |
| 5 | CHOBERKA, Sophia | King's Academy | 1:02.41 | Benjamin Meet #1 |
| 6 | CAMPBELL, Elizabeth | St. Edward's | 1:03.94 | Benjamin Meet #1 2023 |
| 7 | NORDLAND, Emma | Jupiter | 1:07.00 | PBGHS Gator Invitational 2023 |
| 8 | TORRES-MORA, Gabriella | Suncoast | 1:08.93 | Seminole Ridge Hawks Last Chance |