| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | AJAMU, Nathan | Suncoast | NT | NT |
| 4 | CANO, Christian | Oxbridge Academy | NT | 57.0 |
| 5 | DAVIS, Ethan | Olympic Heights | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HOLLIDAY, Jaxson | Olympic Heights | NT | NT |
| 3 | MIYAKE, Caio | Olympic Heights | NT | NT |
| 4 | CENATUS, Ethan | Olympic Heights | NT | NT |
| 5 | STEWART, Tristan | King's Academy | NT | NT |
| 6 | ROSS, Tyler | St. Edward's | NT | 57.0 |
| 7 | SCOTT, Kameron | Suncoast | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | COWAN, Sebastian | Oxbridge Academy | 1:03.64 | Royal Palm Beach Invitational 1 |
| 2 | FISCHER, Ben | King's Academy | 1:01.34 | South Fork Meet #1 |
| 3 | CLARKE, Nathan | Berean Christian | 59.69 | Westminster Academy Invitational |
| 4 | COLLINS, Adrian | Berean Christian | 59.13 | Westminster Academy Invitational |
| 5 | HEIM, Brady | Suncoast | 59.35 | NPB Conference Meet 2023 |
| 6 | SUKHU, Mason | Benjamin School | 1:00.98 | Kings Var Open 2 |
| 7 | DAGGS, Britton | Oxbridge Academy | 1:01.94 | Royal Palm Beach Invitational 2 |
| 8 | BUENO-TAVERAS, Andrew | Suncoast | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GARLAND, Brandon | Benjamin School | 57.15 | Kings Var Open 2 |
| 2 | STINSON, Brian | Berean Christian | 55.92 | Westminster Academy Invitational |
| 3 | ANTEDOMINECO, Ryan | Jupiter | 54.96 | Warrior Invitational 2023 |
| 4 | MACKEY, Jaden | John Carroll | 52.22 | St. Lucie County Meet 2023 |
| 5 | SCHMIDTKE, Sam | King's Academy | 52.33 | FHSAA 2A District 13 |
| 6 | PEREZ, Nicolas | Berean Christian | 55.54 | Westminster Academy Invitational |
| 7 | KAPP, Micah | Oxbridge Academy | 56.60 | Park Vista Meet #1 |
| 8 | HAYDEN, Sebastian | King's Academy | 58.21 | Saint Andrew's HS Track Invitational #2 |