| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jensen Beach |
A | Fischer | Schmidt | Morris | Acheampong | 4:42.16 | Jensen Beach Meet #2 |
| 4 | Benjamin School |
A | Arendts | Morse | Downey | Gay | 4:01.60 | FHSAA 1A District 13 |
| 5 | Martin County |
A | Osdon | Lindsay | Bussom | Hopkins | 4:21.23 | Cobra Relays 2025 |