| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jensen Beach |
A | Peruski | Trimble | Warburton | Bradley | DNF | Jensen Beach Invitational 2026 |
| 4 | Benjamin School |
A | Travers | Bevevino | Sukhu | Travers | DQ | FHSAA State Track & Field Championships (1A) |
| 5 | Martin County |
A | Fernandes | Perez | Osdon | Ebanks | 50.18 | FHSAA 4A Region 2 |