| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pine School |
A | Anderson | Hughes | Bush | Leighty | 4:04.00 | Dr. Garcia Meet #1 |
| 3 | Clewiston |
A | James | mckenzie | mullings | Givens | DNS | FHSAA 2A District 12 |
| 4 | Benjamin School |
A | Reddick | Faniel | Noble | Searcy | 3:23.56 | Benjamin Invite #1 2025 (Sanctioned) |
| 5 | Martin County |
A | Dennis | St. Juste | McLean | Allison | 3:33.52 | FHSAA 4A Region 2 |
| 6 | Jensen Beach |
A | Megibow | Huston | Huston | Avril | 3:55.24 | FHSAA 3A District 13 |