| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Benjamin School |
A | D'Loughy | Carlson | Robbins | Klein | 8:11.42 | FHSAA State Track & Field Championships (1A) |
| 2 | Jensen Beach |
A | Phillips | Vancour | Beck | Glover | 8:38.34 | Reggie Johnson Invitational 2026 |
| 3 | Clewiston |
A | Segura | Exilhomme | mullings | Bussott | 9:06.80 | FHSAA 2A Region 3 |