| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Edward's |
A | Hebig | Moylan | Weber | Storts | 5:10.00 | |
| 3 | John Carroll |
A | Martinez | Ward | Tokman | Fulmer | 4:26.78 | FHSAA 1A Region 4 |
| 4 | Benjamin School |
A | Marshall | Lewis | Downey | Balistreri | DNS | FHSAA State Track & Field Championships (1A) |
| 5 | Spanish River |
A | Ulysse | Theissen | Fray | Popescu | 4:21.44 | Jeff Fossen Invitational 2024 |
| 6 | Suncoast |
A | Johnson | Felin | Herron | Bright | DNS | Dr. Joaquin Garcia Bulldog Distance Jamboree 2025 |