| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Spanish River |
A | Arnold | Perez | Kubinec | Schlesinger | 9:37.35 | FHSAA 4A Region 3 |
| 2 | Benjamin School |
A | Hill | Lewis | Travers | Balistreri | 10:40.48 | Palm Beach County Championships |
| 3 | Atlantic Christian |
A | Santana | Stuart | Almanza | James | 10:44.66 | FHSAA 1A Region 4 |
| 4 | Jupiter |
A | Hanssen | Godinho | O'Shea | Ulmer | 10:44.73 | Dr Garcia Invite #1 2025 |
| 5 | St. Edward's |
A | Callaghan | Cooper | Storts | Perrin | 10:59.17 | Viera High School Invitational |