| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | WEDA, BENJAMIN | Jupiter Christian | NT | |
| 4 | GUNICHEV, Julian | Atlantic Christian | NT | |
| 5 | CALDWELL, William | Jupiter Christian | NT | |
| 6 | SINCAVAGE, Dean | St. Edward's | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MORA, Blake | Olympic Heights | NT | |
| 2 | PLAISIR, Amedstone | Dreyfoos | 1:02.20 | St Andrews Varsity Open 1 |
| 3 | CHANDLER, Grayson | Atlantic Christian | 1:00.62 | St Andrews Varsity Open 1 |
| 4 | HAYNES, Julian | Olympic Heights | 58.70 | Jeff Fossen Memorial Meet 2023 |
| 5 | POITIER, Gabriel | Atlantic Christian | 59.24 | Jeff Fossen Memorial Meet 2023 |
| 6 | AQUEL-LACAYO, Elias | Dreyfoos | 1:01.50 | |
| 7 | BUONOMO, Max | Dreyfoos | 1:06.18 | PBC Freshman Sophomore Meet 2023 |
| 8 | SISCA, ANTHONY | Jupiter Christian | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DOMINIQUE, Wichensky | Atlantic Christian | 57.84 | St Andrews Varsity Open 1 |
| 2 | RYBA, Baron | Benjamin School | 55.00 | |
| 3 | DAVIS, Koen | Dreyfoos | 52.95 | Seminole Ridge Hawks Last Chance |
| 4 | MAYS, Micah | Benjamin School | 47.52 | FHSAA Class 1A Track & Field State Championships |
| 5 | GRANDE, Cole | Benjamin School | 51.00 | |
| 6 | LOUIS, Temani | Olympic Heights | 54.98 | Benjamin Meet #1 |
| 7 | BURREL, Luke | Olympic Heights | 55.26 | Jeff Fossen Memorial Meet 2023 |
| 8 | JOHNSON, Jay'Vier | Benjamin School | 58.00 |