| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Douglas |
A | Anzures | Gago | Bayarri | Hokanson | 4:51.35 | BCAA Regular at South Plantation |
| 3 | Stranahan |
A | Morgan | Seide | Reaves | Johnson | 4:41.52 | FHSAA 3A District 15 |
| 4 | Coral Glades |
A | Chester | Joseph | Sanon | De Belen | 4:17.22 | FHSAA 4A District 13 |
| 5 | Coral Springs Charter |
A | Manigat | Garbizo | Richardson | Schirripa | 4:32.58 | FHSAA 2A District 13 |
| 6 | Coral Springs Charter |
B | Thomas | Parrott | Maniscalco | Sawyer | 4:45.00 |