| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MITCHELL, Josiah | Sebring |
56.55 | Mike Williams Classic @ Lake Region 2025 |
| 2 | MYRICK, Bryan | Hardee |
55.53 | Sebring Meet #2 2025 |
| 3 | WATKINS, Jason | Avon Park |
54.40 | Frostproof - The Ridge Open 2025 |
| 4 | ANDERSON, D'Ylyn-Nikym | Lake Placid |
53.28 | Lake Placid Invite |
| 5 | WALTON, Terrell | Lake Placid |
54.30 | Avon Park Meet #1 2025 |
| 6 | LACOSSADE, Juvel | Vanguard (LW) |
55.39 | Sebring Meet #2 2025 |
| 7 | THOMPSON, Micha | Hardee |
55.94 | Sebring Meet #2 2025 |
| 8 | BRUNSON, Elijah | Hardee |
56.69 | Larry Richardson Memorial Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TICE, I'varion | DeSoto County |
NT | NT |
| 2 | SEVERO, Alexander | Avon Park |
1:01.62 | Hardee Invitational |
| 3 | TIMMONS, Jahari | Avon Park |
1:00.26 | Lake Placid Invite |
| 4 | QUINONES, Christian | DeSoto County |
57.17 | Hardee Invitational |
| 5 | HUGHES, Ezell | Hardee |
59.01 | Hardee Invitational |
| 6 | COHEN, Diangelo | Avon Park |
1:01.20 | Sebring Meet #2 2025 |
| 7 | MORGAN, Luc | DeSoto County |
1:03.89 | Avon Park Meet #1 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | SEYMOUR, Josiah | Vanguard (LW) |
1:11.82 | Sebring Meet #2 2025 |
| 5 | SHANNON, Favid | Sebring |
NT | 56.00 |