| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BOWES, Melina | Hardee |
21.02 | Mike Williams Classic @ Lake Region 2025 |
| 3 | PIERRE, Danaelle | Hardee |
18.74 | Larry Richardson Memorial Invitational |
| 4 | WATT, Kamila | Avon Park |
17.26 | Lake Placid Invite |
| 5 | JOHNSON, Mahagony | Avon Park |
17.63 | Lake Placid Invite |
| 6 | JACKSON, Alexandra | Hardee |
19.64 | Larry Richardson Memorial Invitational |
| 7 | DESTINO, Zarmiah | Avon Park |
21.08 | Sebring Meet #2 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DOBSON, Shamiya | Avon Park |
NT | NT |
| 3 | SHELTON, Marissa | Hardee |
23.31 | Hardee Invitational |
| 4 | PADRON, Anahi | DeSoto County |
21.46 | Immokalee Last Chance |
| 5 | WHITE, Sa'Niyah | DeSoto County |
22.65 | Sebring Meet #2 2025 |
| 6 | ZAMORA, Yoselin | DeSoto County |
24.06 | Sebring Meet #2 2025 |