| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BIRT, Kaylynn | Sebring |
NT | 1:14.00 |
| 2 | GREIDER, Caroline | Lake Placid |
1:28.22 | Avon Park Meet #1 2025 |
| 3 | CHAMBERLEIN, Lana | Avon Park |
1:18.87 | Hardee Invitational |
| 4 | SANTIAGO, Gabriela | Lake Placid |
1:17.51 | Hardee Invitational |
| 5 | VIRKLER, Elisa | Avon Park |
1:18.25 | Sebring Meet #2 2025 |
| 6 | DIAZ, Arlene | Lake Placid |
1:25.91 | Lake Placid Invite |
| 7 | HIPOLITO-HERNANDEZ, Natalie | Hardee |
NT | NT |
| 8 | DOBSON, Shamiya | Avon Park |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TREJO-BALDERAS, Camila | DeSoto County |
1:15.35 | Avon Park Meet #1 2025 |
| 2 | VILLA, Maria | Hardee |
1:10.57 | Mike Williams Classic @ Lake Region 2025 |
| 3 | BOWES, Melina | Hardee |
1:06.59 | Sebring Meet #2 2025 |
| 4 | NUNEZ, Alexa | Hardee |
1:01.69 | Sebring Meet #2 2025 |
| 5 | GLABACH, Myra | Avon Park |
1:05.10 | Sebring Meet #2 2025 |
| 6 | HANNON, Jananarra | DeSoto County |
1:09.65 | Sebring Meet #2 2025 |
| 7 | JOHNSON, Ariel | DeSoto County |
1:15.27 | Avon Park Meet #1 2025 |
| 8 | ELABED, Sujud | DeSoto County |
1:16.06 | Sebring Meet #2 2025 |