| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | CAPRON, Kira | Hardee |
16.29 | Lakewood Ranch Invitational 2025 |
| 4 | RAMOS, Esmeralda | Hardee |
15.68 | Sebring Meet #2 2025 |
| 5 | LEGREE, Shatajah | Lake Placid |
15.92 | Avon Park Meet #1 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | MANRIQUEZ, Adela | Hardee |
15.07 | Sebring Meet #2 2025 |
| 3 | REED, Jada | DeSoto County |
14.12 | Immokalee Last Chance |
| 4 | DESTINO, Zarmiah | Avon Park |
14.01 | Sebring Meet #2 2025 |
| 5 | WILEY, Nariah | DeSoto County |
14.03 | Sebring Meet #2 2025 |
| 6 | HOLLEY, Jaeda | Hardee |
14.67 | Sebring Meet #2 2025 |
| 7 | CAMPBELL, Lailah | Lake Placid |
15.08 | Sebring Meet #2 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DAVI, Mia | Avon Park |
13.92 | Lake Placid Invite |
| 2 | DILLON, Makayla | Lake Placid |
13.42 | Lake Placid Invite |
| 3 | WATT, Kamila | Avon Park |
13.04 | Sebring Meet #2 2025 |
| 4 | HENRY, Tomiah | DeSoto County |
12.75 | Sebring Meet #2 2025 |
| 5 | TYLER, Yahri | DeSoto County |
12.98 | Sebring Meet #2 2025 |
| 6 | JENNINGS, Chloe | Vanguard (LW) |
13.04 | Sebring Meet #2 2025 |
| 7 | DOBSON, Amariya | Sebring |
13.82 | Sebring Meet #2 2025 |
| 8 | GREENE, Ja'zariah | Avon Park |
13.99 | Sebring Meet #2 2025 |