| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PADRON, Anahi | DeSoto County |
25.85 | Dunbar Tiger Classic Invite (Approved Qualifier) |
| 3 | TOWNER, Riley | Lake Placid |
21.93 | Avon Park Red Devil Invitational |
| 4 | JOHNSON, Mahagony | Avon Park |
16.41 | Avon Park Red Devil Invitational |
| 5 | DESTINO , Zarmiah | Avon Park |
20.36 | Tenoroc High School Invitational at SEU |
| 6 | WHITE, Sa'Niyah | DeSoto County |
22.16 | Larry Richardson Memorial Invite |
| 7 | SANTIAGO, Sophia | Lake Placid |
NT |