| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | SANTIAGO, Sophia | Lake Placid |
21.49 | Lake Placid Invitational 2026 |
| 4 | PADRON, Anahi | DeSoto County |
22.12 | Avon Park Invitational |
| 5 | PORTER, Serenity | Four Corners |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | TOWNER, Riley | Lake Placid |
20.90 | Lake Placid Invitational 2026 |
| 3 | PIERRE, Danaelle | Hardee |
18.78 | Avon Park Red Devil Invitational |
| 4 | JOHNSON, Mahagony | Avon Park |
16.07 | Avon Park Invitational |
| 5 | WATT, Kamila | Avon Park |
17.52 | Lake Placid Invitational 2026 |
| 6 | DESTINO , Zarmiah | Avon Park |
20.36 | Tenoroc High School Invitational at SEU |
| 7 | WHITE, Sa'Niyah | DeSoto County |
21.16 | Lake Placid Invitational 2026 |
| 8 | SEVERE, Erianna | Hardee |
21.67 | Lake Placid Invitational 2026 |