| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Royal Palm Beach |
A | Bradwell | sainvil | Neptune | Ennis | 4:37.36 | Royal Palm Beach Invitational 2 |
| 3 | Inlet Grove |
A | Gibson | Steward | Harper | Sharpe | 4:25.33 | FHSAA 2A Region 4 |
| 4 | Pahokee |
A | White | Bythwood | Johnson | Flowers | 3:56.04 | FHSAA 1A Region 4 |
| 5 | Somerset College Prep | A | Bourne-Morgan | Wright | Miller | Rodgers | DNS | South Fork Meet #2 2024 |
| 6 | John I. Leonard |
A | Augustin | lapointe | dorceus | graham | DQ | John I Leonard Invitational |
| 7 | Glades Central |
A | Sellers | Frazier | Gillyard | Kyles | DNS | Anquan Boldin Invitational 2024 |