| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | HARYMAN, Giselle | Glades Day |
NT | |
| 4 | POOLE, Paylyn | Glades Day |
21.01 | Pahokee Anquan Boldin Invitational 2025 |
| 5 | HART, Alniyah | Pahokee |
NT | |
| 6 | PORTER, Heraje | Royal Palm Beach |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PIERCE, Kaniah | Glades Central |
20.79 | FHSAA 2A District 13 |
| 2 | FOWLER, Ta'Niyah | Glades Central |
19.55 | John I Leonard Invitational |
| 3 | MINUS, Lawrencia | Glades Central |
18.84 | FHSAA 2A Region 4 |
| 4 | BOURNE-MORGAN, Natasha | Somerset College Prep | 16.02 | FHSAA 2A Region 2 |
| 5 | BAILEY, Denijah | Glades Day |
17.67 | FHSAA 1A Region 4 |
| 6 | PINTO, Winnyia | Glades Central |
19.43 | FHSAA 2A District 13 |
| 7 | HOLMES, Dallas | Pahokee |
19.81 | John I Leonard Invitational |
| 8 | BYTHWOOD, De'Asia | Pahokee |
20.99 | Pahokee Anquan Boldin Invitational 2025 |