| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | ARNOLD, Quanteria | 13 | Harlem Wolves Athletics | NT | |
| 4 | HOLMES, Milan | 14 | Harlem Wolves Athletics | NT | |
| 5 | DAVIS, O'Shiaya | 14 | Lakeland Cardinals | NT | |
| 6 | ROBERTS, Kamisha | 13 | Harlem Wolves Athletics | NT |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | THOMAS, Tra'Nearia | 14 | Florida Thoroughbreds Track Cl | NT | |
| 3 | EVANS, Ja'Riyah | 13 | Pahokee Elite Track | NT | |
| 4 | STEWARD, Netta | 14 | Sweatshop NBS | NT | |
| 5 | BUSH, Ayla | 13 | Treasure Coast Trailblazers G | NT | |
| 6 | RODRIGUEZ, Arisdeily | 13 | Florida Thoroughbreds Track Cl | NT | |
| 7 | FLOWER, Jillesia | 14 | TCBlaze | NT |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIS, Aysia | 13 | Harlem Wolves Athletics | NT | |
| 2 | JOHNSON, Aariah | 14 | Harlem Wolves Athletics | NT | |
| 3 | ABLORDEPPEY, Justyce | 14 | 2Fast Track Club | 29.05 | |
| 4 | WILLIAMS, Aaysia | 14 | Unattached | 26.48 | |
| 5 | SMITH, Johnni | 13 | Indian River Elite Track Club | 28.90 | |
| 6 | MORSE, Telia | 14 | Florida Thoroughbreds Track Cl | NT | |
| 7 | JONES, Ernajsia | 13 | Harlem Wolves Athletics | NT | |
| 8 | OYOLA, Isabella | 14 | 2Fast Track Club | NT |