| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Park Vista | A | 44.71 | 44.71 Park Vista Meet %231 |
| 2 | Olympic Heights | A | 44.25 | 44.25 Palm Beach Conf South at Atlantic |
| 3 | West Boca Raton | A | 43.57 | 43.57 John I Leonard Invitational |
| 4 | Atlantic (Delray) | A | 42.67 | 42.67 Boynton Beach Invitational |
| 5 | Santaluces | A | 43.03 | 43.03 John I Leonard Invitational |
| 6 | Spanish River | A | 44.02 | 44.02 Palm Beach Conf South at Atlantic |
| 7 | Lake Worth | A | 44.37 | 44.37 Dwyer Panther Relays |
| 8 | Boca Raton | A | 45.32 | 45.32 Boca Bobcats Classic Invitational |