| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Benjamin School |
A | Foster | Klein | Himmich | Laurinaitis | 10:13.47 | Benjamin Invite #1 2025 (Sanctioned) |
| 2 | Treasure Coast | A | Bouchfaa | Walters | Swann | Jean | 10:25.06 | FHSAA 4A District 10 |
| 3 | Wellington |
A | Carbocci | Bentley | Lewis | White | 10:28.26 | 28th Annual Warrior Invitational 2024 |
| 4 | Jupiter |
A | Scheid | Godinho | Mateo | Shaw | 10:43.27 | Benjamin Invite #1 2025 (Sanctioned) |
| 5 | Fort Pierce Central |
A | Dougherty | Riascos | Louissaint | Walker | 11:43.18 | Cobra Relays 2025 |