| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Benjamin School |
A | Irish | Flemming | Olbers | Pearsall | 8:02.78 | FHSAA State Track & Field Championships (1A) |
| 2 | Wellington |
A | Smith | Benedetti Alizo | Pierre-Louis | Wroy | 8:15.12 | FHSAA 4A Region 3 |
| 3 | Centennial |
A | Gedeum | Dimarco | Kaegi | Kirkwood | 8:28.10 | Cobra Relays 2025 |
| 4 | Suncoast |
A | Jolicoeur | Cao | Endrud | Streets | 8:28.13 | FHSAA 3A Region 4 |
| 5 | Blanche Ely |
A | Michel | White | Wilkins | Desvallons | 8:42.32 | FHSAA 3A District 14 |
| 6 | Jupiter |
A | Vargas | Fike | Dennis | George | 8:48.59 | FHSAA 4A District 11 |
| 7 | Fort Pierce Central |
A | O'Dwyer | Randolph | Francois | Robinson | 8:55.51 | 28th Annual Warrior Invitational 2024 |