| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MANJAH, Miresa | SO | Milton Hershey | 59.74 | Unverified: |
| 2 | LEPLEY, Riley | SO | Mechanicsburg Area High School | 1:02.31 | Verified |
| 3 | KAPEL, Dahyuah "Brenice" | SR | Milton Hershey | 51.44 | Unverified: |
| 4 | DILLON, Kyra | SR | Mechanicsburg Area High School | 47.56 | Verified |
| 5 | JACKSON, Iris | SR | Milton Hershey | 51.25 | Verified |
| 6 | EVANS, Grace | SO | Mechanicsburg Area High School | 1:01.48 | Verified |
| 7 | ANYIA, Britney | JR | Milton Hershey | 51.96 | Unverified: |
| 8 | RASHFORD, Emma | SO | Mechanicsburg Area High School | 1:04.24 | Unverified: |