| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MANYUON, Yar | JR | Middletown Area | NT | |
| 3 | WILLIAMS, Vera Lynn | FR | West Perry | 1:08.0h | |
| 4 | SCHAAF, Kelsey | SO | Middletown Area | 1:05.83 | East Pennsboro Twilight Invitati |
| 5 | BYERS, Jordan | SR | West Perry | 1:00.56 | Mid Penn Championship |
| 6 | KOUPAR, Janet | JR | Middletown Area | 1:21.03 | Trinity HS vs. Middletown HS |
| 7 | DILLMAN, Addison | FR | West Perry | 1:10.0h |