| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EBERSOLE, Monroe | FR | Red Land | 1:20.10 | |
| 2 | NEWMAN, Gemma | FR | Red Land | 1:09.13 | Hershey HS vs Red Land HS and We |
| 3 | GULA, Chelsea | SO | Lower Dauphin 03 | 1:02.85 | Milton Hershey vs Lower Dauphin |
| 4 | MCKAY, Kendall | SO | Red Land | 1:07.04 | 15th Annual Twin Valley Raider I |
| 5 | BEAN, Charlotte | SR | Lower Dauphin 03 | 1:07.71 | 34th Annual Stan Morgan Invitati |
| 6 | MCNEIL, Aniya | SO | Red Land | 1:17.60 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CHESEK, Lexi | SO | Red Land | NT | |
| 3 | BUCH, Camryn | FR | Red Land | 1:22.00 | |
| 4 | ARCE, Natalia | FR | Red Land | 1:31.00 | |
| 5 | BOWLING, Alexis | SO | Red Land | NT |