| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Harrisburg 03 | A | 4:33.00 | William Penn quad |
| 4 | Greencastle-Antrim 03 | A | 4:28.04 | 37th Pan Ram Invitational |
| 5 | Middletown Area | A | 4:30.40 | East Pennsboro Twilight Invitati |
| 6 | Cedar Cliff 03 | A | 4:34.20 | 65th Annual Cedar Cliff Relays J |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Boiling Springs | A | 4:21.24 | BSHS 2026 |
| 2 | Hershey 03 | A | 4:17.36 | Hoka Invitational @ West Chester |
| 3 | Central Dauphin 03 | A | 4:13.92 | Central Dauphin vs Harrisburg vs |
| 4 | James Buchanan | A | 4:10.24 | |
| 5 | Northern York 03 | A | 4:10.72 | 22nd Annual Hoka Henderson Invit |
| 6 | Shippensburg 03 | A | 4:13.94 | |
| 7 | Central Dauphin East 03 | A | 4:18.98 | William Penn quad |
| 8 | Gettysburg Area 03 | A | 4:26.94 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Trinity 03 | A | 4:06.47 | East Pennsboro Twilight Invitati |
| 2 | Milton Hershey 03 | A | 4:06.08 | Mechanicsburg vs Milton Hershey |
| 3 | Chambersburg 03 | A | 4:01.64 | |
| 4 | Cumberland Valley 03 | A | 3:56.21 | The 130th Penn Relays celebratin |
| 5 | Palmyra 03 | A | 4:00.31 | Lebanon County HS T&F Championsh |
| 6 | Carlisle 03 | A | 4:03.97 | 37th Annual Stan Morgan Invitati |
| 7 | Mechanicsburg Area | A | 4:06.39 | |
| 8 | East Pennsboro Area 03 | A | 4:08.87 | Penn Relays |