| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 104 | WEIDMAN, Jazlyn | FR | Lower Dauphin 03 | 54.60 | Milton Hershey vs Lower Dauphin |
| 3 | 150 | MAYER, Nova | JR | Lower Dauphin 03 | 45.36 | 36th Annual Stan Morgan Invitati |
| 4 | 53 | MCNEAL, Deidre | FR | Cedar Cliff 03 | NT | |
| 5 | 158 | ROBERTSON, Torree | SR | Lower Dauphin 03 | 53.84 | Lower Dauphin HS vs. Mechanicsbu |