| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Annville-Cleona 03 | A | 46.57 | Tim Cook Invitational |
| 4 | York Suburban | A | 46.44 | Herb Schmidt Relays |
| 5 | North Penn - Mansfield 04 | A | 46.54 | Kirby Classic Relays |
| 6 | Susquenita 03 | A | 46.59 | Juniata at Susquenita |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bellefonte Area 06 | A | 45.49 | Bellefonte vs Central, Central C |
| 2 | Trinity 03 | A | 45.08 | Trinity HS vs. Middletown HS |
| 3 | Huntingdon Area 06 | A | 44.87 | Huntingdon/Hollidaysburg/Bellwoo |
| 4 | Gettysburg Area 03 | A | DNS | Arctic Blast Invitational |
| 5 | Chambersburg 03 | A | 44.81 | Chambersburg @ State College |
| 6 | Boiling Springs | A | 45.00 | vs Shippensburg 2026 |
| 7 | Susquehannock | A | 45.37 | |
| 8 | Lower Dauphin 03 | A | 45.8h | Milton Hershey vs Lower Dauphin |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bellwood Antis 06 | A | 44.64 | |
| 2 | Plum 07 | A | 44.50 | 49th Annual Altoona Area Igloo I |
| 3 | Central York 03 | A | 44.08 | |
| 4 | Altoona Area 06 | A | 43.68 | |
| 5 | Fairfield Area 03 | A | 44.00 | Carlisle Invitational |
| 6 | South Western 03 | A | 44.14 | South Western @ Spring Grove |
| 7 | Palmyra 03 | A | 44.58 | Palmyra HS vs. Cedar Cliff HS (a |
| 8 | Easton Area 11 | A | 44.74 | Stroudsburg at Easton |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | North Schuylkill 11 | A | 43.49 | North Schuylkill at Blue Mountai |
| 2 | East Pennsboro Area 03 | A | 43.45 | Tim Cook Invitational |
| 3 | Carlisle 03 | A | 43.37 | Carlisle HS vs. State College HS |
| 4 | Freedom 11 | A | DNF | Nazareth vs Freedom |
| 5 | West Chester Rustin 01 | A | 43.26 | Rustin Invitational |
| 6 | Selinsgrove 04 | A | 43.42 | Don Wilhour Selinsgrove Classic |
| 7 | Danville Area 04 | A | 43.47 | Don Wilhour Selinsgrove Classic |
| 8 | State College 06 | A | 43.50 | Carlisle HS vs. State College HS |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Dallastown 03 | A | 43.08 | Dallastown vs William Penn |
| 2 | Cumberland Valley 03 | A | 43.01 | Bruce Dallas Invitational |
| 3 | Hollidaysburg 06 | A | 42.91 | |
| 4 | Spring Grove 03 | A | 42.84 | |
| 5 | Wilkes-Barre Area 02 | A | 42.90 | |
| 6 | Greencastle-Antrim 03 | A | 42.97 | 37th Pan Ram Invitational |
| 7 | Ephrata 03 | A | 43.05 | Jeff Bradley Invitational |
| 8 | Parkland 11 | A | 43.11 | 2026 Nazareth @ Parkland |