| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ABRAHAM, Ann | SO | Hershey 03 | 56.90 | Milton Hershey vs Hershey |
| 2 | MOHAN, Harini | SO | Hershey 03 | 55.42 | Hershey HS vs Milton Hershey HS |
| 4 | CAPLES, Claire | SO | Hershey 03 | 56.38 | Milton Hershey vs Hershey |
| 5 | HITCHCOCK, BiƱya | FR | Hershey 03 | 49.61 | The Miller Sports Construction B |
| 6 | SIDER, Eli | JR | Hershey 03 | 51.81 | 17th Cy Fritz Open @ Millersvill |
| 7 | COMMISSIONG, Kennedy | JR | Hershey 03 | 55.50 |