| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | DEGRAFF, Grace | SO | Hershey 03 | 1:05.96 | Hershey HS vs. Lower Dauphin HS |
| 3 | COMMISSIONG, Kennedy | JR | Hershey 03 | 1:02.83 | 2023 USATF National Youth Outdoo |
| 4 | SHIRK, Tyisha | FR | Susquehanna Township 03 | 1:10.31 | The Miller Sports Construction B |
| 5 | ENDLICH, Madeline | SR | Hershey 03 | 1:01.29 | Hershey HS vs Mechanicsburg |
| 6 | SLAPPY, Sarai | FR | Susquehanna Township 03 | 1:17.39 | Susquehanna Twp. Vs. Cedar Cliff |
| 7 | KIM, Sophia | SR | Hershey 03 | 1:03.10 | |
| 8 | ACEVEDO, Eva | Susquehanna Township 03 | NT |