| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 91 | HILL-VEREEN, Maelee | FR | Shippensburg 03 | 57.10 | |
| 3 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 3 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 3 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 3 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 4 | 133 | RITTER, Macy | SO | Shippensburg 03 | 48.16 | Mid Penn Championship |
| 5 | 80 | SCAVITTO, Gabriella | SO | Gettysburg Area 03 | 57.91 | Gettysburg HS vs. James Buchanan |
| 6 | 101 | ST. CLAIR, Hailey | SR | Shippensburg 03 | 57.20 |