| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 160 | REED, Ella | FR | Shippensburg 03 | 1:08.00 | |
| 2 | 204 | BARD, Taylor | SR | Shippensburg 03 | 1:07.00 | |
| 3 | 78 | CRAWFORD, McKayla | SO | Gettysburg Area 03 | 1:06.60 | Adams County Classic |
| 4 | 140 | RENSHAW, Skylen | SR | Shippensburg 03 | 59.70 | Mid Penn Championship |
| 5 | 40 | GLADFELTER, Kohan | SO | Gettysburg Area 03 | 1:07.89 | AAU Mid-Atlantic Qualifier |
| 6 | 42 | SANDERS, Melina | FR | Gettysburg Area 03 | 1:12.50 | Gettysburg vs Big Spring |