| # | Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|---|
| 1 | 133 | RITTER, Macy | SO | Shippensburg 03 | 48.16 | Mid Penn Championship |
| 2 | 3 | ALBRECHT, Ellie | FR | Gettysburg Area 03 | 54.56 | Gettysburg HS vs. James Buchanan |
| 3 | 91 | HILL-VEREEN, Maelee | FR | Shippensburg 03 | 57.10 | |
| 4 | 101 | ST. CLAIR, Hailey | SR | Shippensburg 03 | 57.20 | |
| 5 | 80 | SCAVITTO, Gabriella | SO | Gettysburg Area 03 | 57.91 | Gettysburg HS vs. James Buchanan |
| 6 | 153 | THOMAS, Maeliah | SO | Shippensburg 03 | 1:01.00 | |
| 7 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 8 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT | |
| 9 | 66 | BROWN, Layla | FR | Gettysburg Area 03 | NT |