| Team | Squad | Seed | Note | |
|---|---|---|---|---|
| 1 | Carlisle 03 | A | 4:05.10 | |
| 2 | Milton Hershey 03 | A | 4:15.65 | Tim Cook Invitational |
| 3 | Mechanicsburg Area | A | 4:23.11 | Mechanicsburg vs Lower Dauphin |
| 4 | Red Land | A | 4:34.14 | Hershey HS vs Red Land HS and We |
| 5 | Cedar Cliff 03 | A | 4:36.97 | 37th Pan Ram Invitational |
| 6 | Milton Hershey 03 | B | NT |