| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARDEN, Chloe | FR | Carlisle 03 | NT | |
| 2 | YATES, Aanesti | JR | Harrisburg 03 | 1:06.23 | Susquehanna Township vs. Mechani |
| 3 | WOLF, Alexis | JR | Carlisle 03 | 48.71 | 36th Annual Stan Morgan Invitati |
| 4 | AJAYI, Ire | FR | Carlisle 03 | NT | |
| 5 | AUTMAN, Ahrayia | SO | Carlisle 03 | 54.80 | Carlisle @ State College |
| 6 | CORBITT, Kylee | SO | Harrisburg 03 | NT |