| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WINDHOLZ, Grayson | FR | Carlisle 03 | NT | |
| 2 | GABASAN, Rayver | FR | Carlisle 03 | NT | |
| 3 | BALDWIN, Connor | SR | Carlisle 03 | 41.30 | Mid Penn Championship |
| 4 | BROWN, Artimus | FR | Harrisburg 03 | NT | |
| 5 | ABDELRAHIM, Abulkhassim | SO | Carlisle 03 | 45.07 | Carlisle HS vs. Cumberland Valle |
| 6 | WASHINGTON, Dimitrious | SO | Harrisburg 03 | 1:02.17 | Carlisle HS vs. Harrisburg HS |