| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | York Suburban | A | 53.69 | Ray Geesey Track and Field Invit |
| 2 | Delone Catholic | A | 53.54 | Trinity HS vs. Steel-High HS and |
| 3 | Bermudian Springs | A | 52.62 | Adams County Classic |
| 4 | New Oxford | A | 51.82 | 36th Annual Stan Morgan Invitati |
| 5 | Dover | A | 52.24 | |
| 6 | Red Lion | A | 53.30 | Herb Schmidt Relays |
| 7 | Littlestown | A | 53.57 | Adams County Classic |
| 8 | Hanover High School | A | 54.54 | Littlestown |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Susquehannock | A | 51.24 | |
| 2 | York County School of Tech | A | 50.74 | Battle for the Bridge |
| 3 | Central York | A | 49.68 | Herb Schmidt Relays |
| 4 | William Penn | A | 48.94 | |
| 5 | Dallastown | A | 49.05 | Henderson Invitational |
| 6 | South Western | A | 50.34 | Altoona Mt. Lion |
| 7 | West York Area | A | 51.04 | Dover vs West York |
| 8 | Eastern York | A | 51.68 | Battle for Bridge Invitational |