| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | BARTELS, Alesha | SO | West Perry | NT | |
| 2 | ISLA, Boden | SO | West Perry | 55.25 | Middletown HS vs. West Perry HS | 
| 3 | ARGUELLES, Maria Maureen | SR | Trinity 03 | 55.25 | East Pennsboro Twilight Invitati | 
| 4 | ALBRIGHT, Autumn | SR | West Perry | 51.90 | 34th Annual Stan Morgan Invitati | 
| 5 | GILL, Kendra | JR | Trinity 03 | 58.81 | Trinity HS vs. East Pennsboro | 
| 6 | BOLZE, Gabby | FR | West Perry | 59.75 | Middletown HS vs. West Perry HS |