| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACOBY, Megan | SR | Delone Catholic | 29.04 | |
| 2 | WILLIAMS, Jayla | SO | Steelton-Highspire | 31.89 | The Miller Sports Construction B |
| 3 | SCANISH, Nora | JR | Trinity 03 | 26.00 | 36th Pan Ram Invitational |
| 4 | KEMP, Lena | JR | Delone Catholic | 28.94 | |
| 5 | HOLMES, Zhakya | FR | Steelton-Highspire | 33.67 | Bob and Louise Trump Invitationa |
| 6 | WALKER, Avery | FR | Trinity 03 | 29.11 | 36th Pan Ram Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STORCH, Vivienne | SO | Delone Catholic | 31.94 | |
| 2 | RICHARDSON, Saahiya | FR | Steelton-Highspire | NT | |
| 3 | DUPES, Bella | SO | Trinity 03 | 29.47 | Trinity HS vs. Camp Hill HS |
| 4 | WITTMER, Brielle | JR | Delone Catholic | 29.76 | |
| 5 | PRYOR, Desiree | JR | Steelton-Highspire | NT | |
| 6 | FOX, Kensie | FR | Trinity 03 | 29.98 | Northern vs. Trinity |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SPAHR, Eleanor | FR | Trinity 03 | 32.86 | Northern vs. Trinity |
| 3 | HALVORSON, Victoria | FR | Trinity 03 | 30.67 | Northern vs. Trinity |
| 4 | CURLEY, Hazel | FR | Trinity 03 | 31.28 | Northern vs. Trinity |
| 5 | OLIVER, Lily | JR | Trinity 03 | NT | |
| 6 | GUILLAUME, Annisah | SO | Trinity 03 | 34.87 | Trinity HS vs. West Perry HS |