| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hershey | C | Cox | Meholic | Lenzo | Peek | DNF | Hershey HS vs Mechanicsburg HS |
| 2 | Palmyra | B | 44.86 | |||||
| 3 | Hershey | A | Oseghale | Weiler-Timmins | Simmons | Sweeney | 44.20 | Hershey |
| 4 | Palmyra | A | Weldon | McDannell | Burgess | Burgess | 41.23 | Tim Cook Invitational |
| 5 | Hershey | B | Poger | Smith | Smith | Lenzo | 45.20 | Hershey HS vs Mechanicsburg HS |
| 6 | Palmyra | C | 50.00 |