| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LUA, Dulce | FR | Bermudian Springs | 59.8h | Hanover |
| 2 | GILL, Kendra | JR | Trinity 03 | 57.40 | Trinity HS vs. West Perry HS |
| 3 | ROMAIN, Gabriella | SO | Middletown Area | 56.53 | Milton Hershey HS Quad Meet |
| 4 | SNYDER, Breelyn | SR | Bermudian Springs | 53.1h | Littlestown |
| 5 | ARGUELLES, Maria Maureen | SR | Trinity 03 | 55.25 | East Pennsboro Twilight Invitati |
| 6 | TOTI, Kaitlyn | FR | Bermudian Springs | 56.28 | Bruce Dallas Invitational |
| 7 | LUA, Jessica | SO | Bermudian Springs | 1:08.5h | Hanover |
| 8 | CARPER, Vivian | SO | Middletown Area | NT |